Aeromonas hydrophila Colony Morphology on CLED Agar

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Aeromonas hydrophila, when cultured on CLED (Cystine-Lactose-Electrolyte-Deficient) agar, exhibits certain distinctive colony morphology characteristics. These characteristics are useful for the identification and differentiation of this bacterium from other microorganisms. Here are some key features of Aeromonas hydrophila colonies on CLED agar:

Colony Color: The colonies typically appear as a creamy or yellowish hue. This is due to the fermentation of lactose present in the CLED agar.

Colony Size: They usually form medium to large-sized colonies, which can be a helpful distinguishing feature.

Colony Shape: The colonies of Aeromonas hydrophila are generally round or circular with a smooth margin.

Colony Texture: They tend to have a smooth, moist, and sometimes mucoid texture, giving them a distinctive appearance.

Hemolysis: Aeromonas hydrophila is not typically known for hemolysis on CLED agar, as this medium does not contain blood.

Growth Pattern: The bacteria may show a rapid growth pattern, becoming noticeable within 24 hours of incubation.

Opacity: The colonies are usually opaque or translucent.

Other Features: On CLED agar, which is designed to prevent the swarming of Proteus species, Aeromonas hydrophila can be more easily isolated and identified.

It's important to note that while these characteristics are typical, variations can occur, and additional tests are usually conducted for a definitive identification of Aeromonas hydrophila. CLED agar is particularly useful in urinary tract infection studies, as it supports the growth of a wide range of urinary pathogens, including Aeromonas species, and inhibits the swarming of Proteus.
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