
He began his animation career in 1925 working for Bray Productions on the Dinky Doodle series, produced under the supervision of Walter Lantz. After Bray he served as an inker on Ben Harrison’s and Manny Gould’s Krazy Kat cartoons before moving to Fleischer Studios in 1929 after producer Charles Mintz did not retain him upon transferring the studio to Hollywood.
Culhane is known for promoting the animation talents of his inker/assistant at Fleischer in the early 1930s, Lillian Friedman Astor, making her the first female studio animator.
After serving as director on several Talkartoons and early Betty Boop shorts, Culhane moved to Hollywood to animate at the Iwerks Studio, operated by influential former Disney alumnus Ub Iwerks, under which he directed, alongside his longtime colleague and friend Al Eugster, several ComiColor Cartoons.
On departing Iwerks's studio, Culhane briefly returned to New York to direct at the reorganized Van Beuren Corporation, then supervised by Burt Gillett, before opting to apply to Disney in 1935.
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