Enterobacter cloacae complex on CLED agar of urine culture

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On a CLED agar plate, Enterobacter cloacae complex will typically appear as pink to red colonies due to their ability to ferment lactose. These colonies may also have a characteristic mucoid appearance. CLED agar is a differential medium that helps to differentiate between lactose-fermenting and non-lactose-fermenting bacteria in urine cultures.
Elaboration:
CLED Agar:
CLED (Cystine Lactose Electrolyte Deficient) agar is a selective and differential medium used for the culture of urine samples.
Lactose Fermentation:
E. cloacae complex is a lactose fermenter, meaning it can break down lactose into simpler sugars. This fermentation results in the production of acids, which are detected by the pH indicator in the CLED agar, causing a color change.
Colony Appearance:
On CLED agar, Enterobacter cloacae complex usually forms pink to red colonies. The red color is due to the acid production from lactose fermentation.
Mucoidy:
Some species within the E. cloacae complex, particularly Enterobacter hormaechei, are known to be mucoid. This means they produce a layer of polysaccharide mucus on their surface, which can make their colonies appear slimy or glistening.
Other Considerations:
While the pink to red color and mucoid appearance are characteristic, other species within the E. cloacae complex may exhibit slightly different colony morphologies on CLED agar.
Clinical Significance:
The isolation of Enterobacter cloacae complex from urine cultures can indicate a urinary tract infection. Further identification and antibiotic susceptibility testing are usually performed to determine the appropriate treatment.
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