Pseudomonas fluorescens under UV light

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Pseudomonas fluorescens, and related species like P. aeruginosa, exhibit fluorescence under UV light due to the production of pigments like pyoverdine, which is a green, iron-scavenging siderophore.
Here's a more detailed explanation:
Fluorescence Mechanism:
The fluorescence occurs because these pigments absorb UV light and then re-emit it as visible light.
Pigments:
Pyoverdine: This is a key pigment responsible for the yellow-green or blue-green fluorescence observed under UV light.
Pyocyanin: Another pigment that can contribute to the bluish-green fluorescence.
Iron Scavenging:
Pyoverdine, in particular, is a siderophore, meaning it helps the bacteria acquire iron, which is essential for bacterial growth.
UV light wavelengths:
The fluorescence is most prominent when exposed to UV light wavelengths around 360 nm.
Identification:
The fluorescence under UV light can be used as a diagnostic tool to help identify Pseudomonas species, especially P. aeruginosa.
Ecological Roles:
P. fluorescens and related species play various roles in soil and plant health, including nutrient cycling and the production of plant-growth-promoting substances.
Disease:
Some strains of P. fluorescens can cause disease in plants, and certain strains can be opportunistic pathogens in humans, particularly in immunocompromised individuals.
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