Automatic Lyophilizer Machine for RT PCR Master Mix | IVD Equipment | Lyophilization Process in IVD

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Automatic Lyophilizer Machine for RT PCR Master Mix | IVD Equipments | Lyophilization Process in IVD@ivdmanufacturing7208

What is Lyophilization?
Lyophilization vs. Freeze Drying
What is lyophilization? How does it work?

Lyophilization and freeze drying are synonymous. Lyophilization is a water removal process typically used to preserve perishable materials, to extend shelf life or make the material more convenient for transport. Lyophilization works by freezing the material, then reducing the pressure and adding heat to allow the frozen water in the material to sublimate.

Lyophilization’s 3 Primary Stages
Lyophilization occurs in three phases, with the first and most critical being the freezing phase. Proper lyophilization can reduce drying times by 30%.



Freezing Phase
There are various methods to freezing the product. Freezing can be done in a freezer, a chilled bath (shell freezer) or on a shelf in the freeze dryer. Cooling the material below its triple point ensures that sublimation, rather than melting, will occur. This preserves its physical form.

Lyophilization is easiest to accomplish using large ice crystals, which can be produced by slow freezing or annealing. However, with biological materials, when crystals are too large they may break the cell walls, and that leads to less-than-ideal freeze drying results. To prevent this, the freezing is done rapidly. For materials that tend to precipitate, annealing can be used. This process involves fast freezing, then raising the product temperature to allow the crystals to grow.



Primary Drying (Sublimation) Phase
Lyophilization’s second phase is primary drying (sublimation), in which the pressure is lowered and heat is added to the material in order for the water to sublimate. The vacuum speeds sublimation. The cold condenser provides a surface for the water vapor to adhere and solidify. The condenser also protects the vacuum pump from the water vapor. About 95% of the water in the material is removed in this phase. Primary drying can be a slow process. Too much heat can alter the structure of the material.



Secondary Drying (Adsorption) Phase
Lyophilization’s final phase is secondary drying (adsorption), during which the ionically-bound water molecules are removed. By raising the temperature higher than in the primary drying phase, the bonds are broken between the material and the water molecules. Freeze dried materials retain a porous structure. After the lyophilization process is complete, the vacuum can be broken with an inert gas before the material is sealed. Most materials can be dried to 1-5% residual moisture.


लियोफिलाइजेशन, लियोफिलाइजर, लियोफिलाइजेशन मशीन, लियोफिलाइजर मशीन, लियोफिलाइजेशन प्रक्रिया क्या है, फ्रीज सुखाने की प्रक्रिया, ड्रग लियोफिलजर, लियोफिलाइजेशन प्रक्रिया क्या होता है, लियोफिलाइजर कैसे ऑपरेट करें, लियोफिलाइजेशन के फायदे



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