Simulating In-Fusion Cloning In SnapGene

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In this tutorial, I will show you how to simulate Takara's In-Fusion cloning with a single fragment in SnapGene.

In-Fusion cloning is a ligation-independent cloning technique that uses complementary annealing of an insert and vector. Briefly, PCR primers are designed to generate flanking homologous overlaps, each around 15-20 bp long, between the cloning insert and vector. The vector and insert products are then recombined with the In-Fusion enzyme.

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